Downstream processing

Downstream processing is the part of a bioprocess where the cell mass from the upstream is processed to meet purity and quality requirements. The isolation and purification of a biotechnological product to a form suitable for its intended use, is termed DSP. Typically, this means recovery of a product from an aqueous solution. The products of biotechnology include whole cells, organic acids, amino acids, solvents, antibiotics, industrial enzymes, therapeutic proteins, vaccines etc. As these products vary greatly in size and nature, different separation principles are required for their recovery and purification.

Media Preparation

Using specific recipes required for each bioreactor stage of scale up from inoculum to harvest. Media preparation is usually carried out in tanks, carboys, bottles or bags to which the media is introduced. Similar to humans, cells also require a proper nutrition to function and ultimately produce the protein product.

Therefore, media is predominantly made up of various components: carbohydrate (glucose), nitrogen (amino acids), fats (lipids) and trace amounts of salt. Media components are most often in powder form which is introduced to a high purity grade of water for injection (WFI). Prior to introducing...

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Cell Culture

Cell culture is the growth of cells outside the body (in vitro). All living things grow by cellular division and same applies to cell culture, the cells divide and continue to multiply once in a suitable environment.

The correct environment consists of a growth medium as a food source and cell culture vessels for controlling gases and temperature. During the growth stage, the medium will turn an opaque colour as the cells density increases. The growth rate is monitored accurately to determine how well cell...

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Cell Separation

Cell Separation is the first step at recovering the protein product (cells) from the culture. The timing for termination of the Cell Culture is crucial and predetermined based on the quality and quantity of the product accumulated in the bioreactors.

The first step in mammalian separation is usually centrifugation: a technique used for the separation of cells from culture through rapid spinning and sedimentation. The technique of centrifugation is based on the principle of density difference between particles to be separated and the medium...

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Downstream

We pack components in custom quantities; a combination of products as specified by the customer, so that they have a ready-to-use package with exactly the components they need for a specific production batch. When specific components are needed, it is useful that an orderly method can be used in the cleanroom, clearly indicating which bag belongs to which. This contributes to the flexibility of production processes and makes work easier for the operator.

How many different production processes do you have? We look beyond the delivered assembly, by considering the final production processes for the operator. For example, supplying very specific numbers and irradiating the range of single use components and assemblies.